The effect of Gallium Aluminium Arsenide laser on fibroblast activity: An in vitro dosimetry study

The effect of Gallium Aluminium Arsenide laser on fibroblast activity: An in vitro dosimetry study

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The effect of different doses of low intensity laser therapy (L.I.L.T.

) on human fibroblasts was investigated to determine the optimal dose required to stimulate fibroblast proliferation.Human fibroblasts were cultured in vitro and irradiated with different energy densities of 83Onm continuous output infra-red laser using a Canvas Beach Bag With Rope Handle Gallium Aluminium Arsenide laser.The fibroblasts were irradiated on three consecutive days at energy densities, ranging from 0.2 to 5 J.

cm2, delivered at an average radiant power of 30 mW, and at a constant distance of lcm from the fibroblasts.Fibroblast activity was assessed on the fourth day using a calorimetric MTT (tetrazolium) cleavage assay.There DSLR Lens was a significant increase in fibroblast proliferation at laser treatment energy densities of 0.4 J.

cm2 and 5 J.cm2.Difficulties associated with in vivo and in vitro studies of the effect of laser treatment are discussed.